- Reliable; optically encoded circuit for setting injection time
- Reproducible
- Easy to use
- Femtoliter to microliter injections
- Digital readouts for injection count, time, and pressure
- 5 pressures: inject, balance, clear, fill, and hold
- Popular applications:
- Injection of mouse, frog, zebrafish and other oocytes
- Extracellular brain injections
- Injection of DNA, mRNA, microbeads, neurotransmitters, kinases and other proteins
Whether you need to do large injections into capillaries or very small injections into mammalian nuclei, the PLI-100A is up to the task.
Pressure to the pipette is controlled precisely through a multi-turn regulator and is reported digitally for reproducibility. Injection time is set using an optically encoded circuit that permits both fine and coarse settings from a single control knob. System timing can be controlled in three ways: push button, foot switch, or external trigger.
The PLI-100A features three positive and two negative pressures.
The three positive functions allow the user to:
- Inject - Inject picoliter to nanoliter amounts of fluids
- Balance - Apply a balance pressure to prevent backflow into the micropipette following an injection
- Clear - Clear a micropipette of material that is blocking the tip
The two negative (or vacuum) functions allow the user to:
- Fill - Fill micropipettes from their tips
- Hold - Provide a means to secure and manipulate a cell using holding pipette.
Unique Features
The PLI-100A features three positive and two negative pressures.
Balance
In addition to the injection pressure, the PLI-100A offers a secondary balance pressure. This adjustable control applies a positive pressure to the injection pipette both before and after an injection that eliminates dilution caused by capillary action and aids in the prevention of clogging. Wasteful continuous injections that can occur when no separate balance pressure is available is avoided.
Fill/Hold
The PLI-100A has two separate vacuum generators used to fill a micropipette and to hold suspended cells while injecting. Filling of the pipette from the tip is less complicated than back-filling. The PLI-100A features a Fill Vacuum adjustment on the front panel.
Hold
Suspended cells can be held in place using a second (holding) pipette. The holding vacuum range accommodates most cell types.
Clear
A high-pressure pulse can be used for clearing a pipette should it happen to clog. This is especially useful when working with pipettes sized for smaller volumes.
Electrical Connectors
BNC type connectors are available at the front panel to ease integrating the PLI with other equipment. Synchronization with or control from injections with a data acquisition system is possible.
Selection Guideline
The PLI-100A is available in three packages: Basic, Plus, and Deluxe:
The Basic unit is supplied with an input hose, output hose, holding hose, and input hose adapter.
The Plus unit also includes a foot switch, pipette holder, and input hose adapter.
The Deluxe unit includes all the accessories included with the Plus and an additional foot switch and pipette holder.
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