Rapp OptoElectronic


ROI Illumination System for Photomanipulation

The UGA-40-GEO is a programmable illumination system designed to illuminate regions of interest (ROI) of different shapes and sizes without scanning. Similar to the UGA-40, the positioning of the spot in the field of view is done by means of fast galvanometer scanners. The system is available for most major microscope models and is compatible with confocal units.


  • programmable, computer controlled illumination of ROIs. More than 40 standard shapes and sizes. Customization available upon request.
  • real-time photomanipulation via the “click and fire” mode
  • sequential illumination of ROIs with user defined positioning and temporal settings
  • simultaneous spot illumination and imaging data acquisition in both the "click & fire" and the "sequence" modes
  • TTL synchronization of sequences or sequence elements with other equipment
  • analog control of laser intensity (when used with lasers provided by Rapp OptoElectronic)
  • high versatility and adaptability

  • Add-on module, can be integrated into existing set-ups
  • Two connections for fiber coupled lasers, one for large spot generation and one for point spots
  • Compatible with laser combiners
  • Switching time between any two spot locations < 1ms
  • Scanning speed within an ROI up to 5kHz
  • 60 different standard shapes and sizes. Customizable upon request.
  • Wide variety of UV, VIS and NIR lasers available (266 to 1535 nm)
  • Switching time between two laser lines: < 1ms
  • Can be combined with wide field fluorescence, LSM and spinning disc confocal, etc.
  • Modules available for most modern research microscope brands and models
  • UGA-40 GEO software runs in parallel with and independent of any other imaging or electrophysiology software
  • Simultaneous and independent spot illumination and imaging data acquisition
  • "Click & Fire" mode for real time spot illumination
  • Programmable illumination (sequences) of multiple locations and/or regions of interests (ROIs)
  • Spot geometries include circles, squares, rectangles and donuts
  • ROI geometries include: lines, circles, polygons, free-hand drawn shapes
  • Grid stimulation with sequential or random illumination of grid points
  • TTL-in and -out
  • TTL-triggered sequences
  • TTL-triggered events within a sequence
  • Able to run multiple lasers within the same experiment